Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.
Identifieur interne : 004677 ( Main/Exploration ); précédent : 004676; suivant : 004678Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.
Auteurs : Aleksandar Radoni [Allemagne] ; Stefanie Thulke ; Hi-Gung Bae ; Marcel A. Müller ; Wolfgang Siegert ; Andreas NitscheSource :
- Virology journal [ 1743-422X ] ; 2005.
Descripteurs français
- KwdFr :
- Cytomegalovirus (génétique), Cytomegalovirus (isolement et purification), Gènes viraux (génétique), Herpèsvirus humain de type 6 (génétique), Herpèsvirus humain de type 6 (isolement et purification), Humains, Lignée cellulaire, Normes de référence, Peptidylpropyl isomerase (génétique), Peptidylpropyl isomerase (métabolisme), Poxviridae (génétique), Poxviridae (isolement et purification), Protéine de liaison à la boite TATA (métabolisme), Réaction de polymérisation en chaîne (), Réaction de polymérisation en chaîne (normes), Réplication virale, Virus de la fièvre jaune (génétique), Virus de la fièvre jaune (isolement et purification), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- génétique : Cytomegalovirus, Gènes viraux, Herpèsvirus humain de type 6, Peptidylpropyl isomerase, Poxviridae, Virus de la fièvre jaune, Virus du SRAS.
- isolement et purification : Cytomegalovirus, Herpèsvirus humain de type 6, Poxviridae, Virus de la fièvre jaune, Virus du SRAS.
- métabolisme : Peptidylpropyl isomerase, Protéine de liaison à la boite TATA.
- normes : Réaction de polymérisation en chaîne.
- Humains, Lignée cellulaire, Normes de référence, Réaction de polymérisation en chaîne, Réplication virale.
English descriptors
- KwdEn :
- Cell Line, Cytomegalovirus (genetics), Cytomegalovirus (isolation & purification), Genes, Viral (genetics), Herpesvirus 6, Human (genetics), Herpesvirus 6, Human (isolation & purification), Humans, Peptidylprolyl Isomerase (genetics), Peptidylprolyl Isomerase (metabolism), Polymerase Chain Reaction (methods), Polymerase Chain Reaction (standards), Poxviridae (genetics), Poxviridae (isolation & purification), Reference Standards, SARS Virus (genetics), SARS Virus (isolation & purification), TATA-Box Binding Protein (metabolism), Virus Replication, Yellow fever virus (genetics), Yellow fever virus (isolation & purification).
- MESH :
- chemical , genetics : Peptidylprolyl Isomerase.
- genetics : Cytomegalovirus, Genes, Viral, Herpesvirus 6, Human, Poxviridae, SARS Virus, Yellow fever virus.
- isolation & purification : Cytomegalovirus, Herpesvirus 6, Human, Poxviridae, SARS Virus, Yellow fever virus.
- chemical , metabolism : Peptidylprolyl Isomerase, TATA-Box Binding Protein.
- methods : Polymerase Chain Reaction.
- standards : Polymerase Chain Reaction.
- Cell Line, Humans, Reference Standards, Virus Replication.
Abstract
Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, beta-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.
DOI: 10.1186/1743-422X-2-7
PubMed: 15705200
Affiliations:
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Le document en format XML
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Hämatologie/Onkologie, Berlin, Germany. aleksandar.radonic@charite.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - CCM, Medizinische Klinik II m.S. Hämatologie/Onkologie, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Thulke, Stefanie" sort="Thulke, Stefanie" uniqKey="Thulke S" first="Stefanie" last="Thulke">Stefanie Thulke</name></author><author><name sortKey="Bae, Hi Gung" sort="Bae, Hi Gung" uniqKey="Bae H" first="Hi-Gung" last="Bae">Hi-Gung Bae</name></author><author><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A" last="Müller">Marcel A. Müller</name></author><author><name sortKey="Siegert, Wolfgang" sort="Siegert, Wolfgang" uniqKey="Siegert W" first="Wolfgang" last="Siegert">Wolfgang Siegert</name></author><author><name sortKey="Nitsche, Andreas" sort="Nitsche, Andreas" uniqKey="Nitsche A" first="Andreas" last="Nitsche">Andreas Nitsche</name></author></analytic><series><title level="j">Virology journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line</term><term>Cytomegalovirus (genetics)</term><term>Cytomegalovirus (isolation & purification)</term><term>Genes, Viral (genetics)</term><term>Herpesvirus 6, Human (genetics)</term><term>Herpesvirus 6, Human (isolation & purification)</term><term>Humans</term><term>Peptidylprolyl Isomerase (genetics)</term><term>Peptidylprolyl Isomerase (metabolism)</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase Chain Reaction (standards)</term><term>Poxviridae (genetics)</term><term>Poxviridae (isolation & purification)</term><term>Reference Standards</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>TATA-Box Binding Protein (metabolism)</term><term>Virus Replication</term><term>Yellow fever virus (genetics)</term><term>Yellow fever virus (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cytomegalovirus (génétique)</term><term>Cytomegalovirus (isolement et purification)</term><term>Gènes viraux (génétique)</term><term>Herpèsvirus humain de type 6 (génétique)</term><term>Herpèsvirus humain de type 6 (isolement et purification)</term><term>Humains</term><term>Lignée cellulaire</term><term>Normes de référence</term><term>Peptidylpropyl isomerase (génétique)</term><term>Peptidylpropyl isomerase (métabolisme)</term><term>Poxviridae (génétique)</term><term>Poxviridae (isolement et purification)</term><term>Protéine de liaison à la boite TATA (métabolisme)</term><term>Réaction de polymérisation en chaîne ()</term><term>Réaction de polymérisation en chaîne (normes)</term><term>Réplication virale</term><term>Virus de la fièvre jaune (génétique)</term><term>Virus de la fièvre jaune (isolement et purification)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidylprolyl Isomerase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cytomegalovirus</term><term>Genes, Viral</term><term>Herpesvirus 6, Human</term><term>Poxviridae</term><term>SARS Virus</term><term>Yellow fever virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cytomegalovirus</term><term>Gènes viraux</term><term>Herpèsvirus humain de type 6</term><term>Peptidylpropyl isomerase</term><term>Poxviridae</term><term>Virus de la fièvre jaune</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Cytomegalovirus</term><term>Herpesvirus 6, Human</term><term>Poxviridae</term><term>SARS Virus</term><term>Yellow fever virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Cytomegalovirus</term><term>Herpèsvirus humain de type 6</term><term>Poxviridae</term><term>Virus de la fièvre jaune</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptidylprolyl Isomerase</term><term>TATA-Box Binding Protein</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peptidylpropyl isomerase</term><term>Protéine de liaison à la boite TATA</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Humans</term><term>Reference Standards</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Lignée cellulaire</term><term>Normes de référence</term><term>Réaction de polymérisation en chaîne</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, beta-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><noCountry><name sortKey="Bae, Hi Gung" sort="Bae, Hi Gung" uniqKey="Bae H" first="Hi-Gung" last="Bae">Hi-Gung Bae</name><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A" last="Müller">Marcel A. Müller</name><name sortKey="Nitsche, Andreas" sort="Nitsche, Andreas" uniqKey="Nitsche A" first="Andreas" last="Nitsche">Andreas Nitsche</name><name sortKey="Siegert, Wolfgang" sort="Siegert, Wolfgang" uniqKey="Siegert W" first="Wolfgang" last="Siegert">Wolfgang Siegert</name><name sortKey="Thulke, Stefanie" sort="Thulke, Stefanie" uniqKey="Thulke S" first="Stefanie" last="Thulke">Stefanie Thulke</name></noCountry><country name="Allemagne"><region name="Berlin"><name sortKey="Radoni, Aleksandar" sort="Radoni, Aleksandar" uniqKey="Radoni A" first="Aleksandar" last="Radoni">Aleksandar Radoni</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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